Open AccessGYNOGENESIS INDUCTION IN CULTURE OF UNPOLLINATED OVULES OF PUMPKIN
Author(s) -
Н.А. Шмыкова,
Д. В. Шумилина,
В.П. Кушнерева,
Г. А. Химич
Publication year - 2011
Publication title -
ovoŝi rossii
Language(s) - English
Resource type - Journals
eISSN - 2618-7132
pISSN - 2072-9146
DOI - 10.18619/2072-9146-2011-1-28-31
Subject(s) - ovule , biology , botany , hybrid , ovary , embryo , egg cell , horticulture , pollen , microbiology and biotechnology , genetics
The perspective of utilization of in vitro culture of unpollinated ovules for development of dihaploid lines of pumpkin is described in the article. The ovules of five hybrids were cultivated on CBM medium. The following combinations of growth regulators for gynogenesis induction were used: a) 0.2 mg/L of thidiazuron and 0,0001 мкМ of epibrassinolide; b) 2 mg/l of 2,4-D. The frequency of embryo development depended on cultivation medium and genotype. The maximum amount of embryos developed from unpollinated ovules of one ovary was 6-9 ones.