Open AccessIsolation of Mesenchymal Stem Cells from Adipose Tissue
Author(s) -
Harsan Harsan,
Silmi Mariya,
Andi Asadul Islam,
Eka Julianta Wahjoepramono,
Irawan Yusuf
Publication year - 2015
Publication title -
the indonesian biomedical journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.16
H-Index - 4
eISSN - 2355-9179
pISSN - 2085-3297
DOI - 10.18585/inabj.v7i3.181
Subject(s) - adipose tissue , mesenchymal stem cell , immunophenotyping , cd90 , stem cell , biology , stem cell transplantation for articular cartilage repair , microbiology and biotechnology , adult stem cell , cd34 , immunology , endothelial stem cell , flow cytometry , in vitro , genetics , biochemistry
BACKGROUND: In searching for the best source of stem cells, researcher found adipose stem cells as one of the ideal source due to its easiness in harvesting and its potential for differentiating into other cell lineage.METHODS: We isolated stem cells from adipose tissue, cultured and confirmed its immunophenotype using polymerase chain reaction.RESULTS: Cluster of differentiation (CD)44, CD73, CD90, CD105 were expressed, which represent immunophenotype of mesenchymal stem cells. CONCLUSION: Mesenchymal stem cells can be isolated from adipose tissue. KEYWORDS: adipose, mesenchymal stem cells, isolation, immunophenotype