Open AccessMorphometric characterization and classification of vicuña sperm (Vicugna vicugna) using ISAS® CASA-Morph system
Author(s) -
Hernán Cucho,
AUTHOR_ID,
Olger Puelles,
Aydee Meza,
Darwin Urquizo,
Leonidas Coila,
Anthony Valverde
Publication year - 2021
Publication title -
spermova
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.102
H-Index - 1
eISSN - 2308-4928
pISSN - 2223-9375
DOI - 10.18548/aspe/0009.16
Subject(s) - sperm , biology , electroejaculation , acrosome , anatomy , semen , perimeter , sperm motility , mathematics , geometry , botany
The aim of the study was to determine the morphological and morphometric characteristics of the vicuña sperm (Vicugna vicugna), looking for possible subpopulations in its structure. Semen was collected by electroejaculation method from three adult male vicuñas weighing 50.33 ± 2.52 kg, once per animal. The volume, color and concentration, sperm morphology and morphometry were determined. The samples for the analysis of sperm morphology and morphometry were stained with Hemacolor®, and analyzed using the CASA-Morph, Integrated Semen Analysis System (ISAS®v1). Five forms of the vicuña sperm head were distinguished: normal, piriform, long, short and rounded. The morphometric parameters determined were the length, width, area, perimeter, ellipticity, elongation, regularity and rugosity of the vicuña sperm head, percentage of acrosome, head gray level, as well as the width, area, distance and angle of insertion of midpiece of the sperm. In relation to morphology, significant differences (P 0.05). The morphometric variables were distributed in four main components (PCA) called elongation, area, circularity and midpiece width, which explained 84.59% of the total variance. The cluster analysis determined five subpopulations (SP): SP1 grouped small cells of low length, width and area (18.8%); SP2 of sperm of large size, both in area and width of the head (17.38%); SP3 of rounded cells with high values of percentage of acrosome and head gray level (24.04%); SP4 of spermatozoa of intermediate size and elongated, with greater ellipticities and elongation (23.61%); and SP5 of cells of intermediate size and short, with low values of area and length (16.71%).