Open AccessLipid and β-Carotene Production by Rhodosporidium diobovatum Cultured with Different Carbon to Nitrogen Ratios
Author(s) -
Maryam Mirzaie,
S. Siavash Saei-Dehkordi,
David B. Levin
Publication year - 2019
Publication title -
journal of food quality and hazards control
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.18
H-Index - 10
eISSN - 2345-6825
pISSN - 2345-685X
DOI - 10.18502/jfqhc.6.2.956
Subject(s) - nitrogen , food science , oleic acid , chemistry , carotenoid , carbon fibers , carotene , carbon to nitrogen ratio , linoleic acid , chromatography , fatty acid , biochemistry , organic chemistry , materials science , composite number , composite material
Background: In food industry, carotenoids are used as food colorants conferring yellow to red color. This research was designed to study on lipid and β-carotene production by Rhodosporidium diobovatum cultured with different Carbon to Nitrogen (C/N) ratios. Methods: R. diobovatum was cultured in a medium containing 40 g/l glucose (as the carbon source) and different C/N ratios (20, 50, and 80), which were established by adding different amounts of (NH4)2SO4 (3.78, 1.51, and 0.94 g/l) as the source of nitrogen. High performance liquid chromatography, gas chromatography, and microplate reader were used to determine the glucose concentration, lipid production, and β-carotene concentration, respectively. Data were analyzed using IBM SPSS statistics (v. 24). Results: Cultures with a C/N ratio of 50 produced the greatest amount of lipids during 120 h pi. However, lipid synthesis in the first 48 h pi was very low for all three C/N ratios. Analyses of the lipid composition revealed that oleic acid and linoleic acid were the dominant (60%) fatty acids. Cultures with a C/N ratio of 50 also produced the greatest amount of β-carotene. Conclusion: R. diobovatum in the C/N of 50 culture medium resulted in greater concentrations of lipid and β-carotene. Defining the optimum C/N ratio will enable development of optimized bioprocess engineering parameters