Open AccessGenotyping of Echinococcus granulosus Isolates from Human in Khorasan Province, North-Eastern Iran
Author(s) -
Fariba Berenji,
Seyed Aliakbar Shamsian,
Marziyeh Nouri Daloee,
Seyed Hossein Fattahi Masoom,
Elham Moghaddas
Publication year - 2019
Publication title -
iranian journal of parasitology./iranian journal of parasitology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.477
H-Index - 27
eISSN - 2008-238X
pISSN - 1735-7020
DOI - 10.18502/ijpa.v14i1.717
Subject(s) - echinococcus granulosus , internal transcribed spacer , genotyping , biology , polymerase chain reaction , veterinary medicine , genotype , cytochrome c oxidase subunit i , gene , ribosomal rna , mitochondrial dna , zoology , genetics , medicine
Background: Human hydatidosis is endemic in northeastern Iran. The present study aimed to investigate molecular diversity of Echinococcus granulosus isolates collected from human surgically.
Methods: Sixty human hydatid cysts (58 lung cysts and 2 liver cysts) were collected through surgery from Ghaem and Emam Reza hospitals in Mashhad University of Medical Sciences during 2015-2016. Cysts were characterized using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of the internal transcribed spacer 1 (ITS1) gene and sequencing fragments of the genes coding for mitochondrial cytochrome c oxidase subunit 1 (cox1) and NADH dehydrogenase subunit I (nad1).
Results: Overall, 55 out of 60 Echinococcus granulosus cysts (91.6%) were determined as the G1 strain, 4 cases (6.6%) were determined as the G6 strain and 1 sample was not identified.
Conclusion: Although sheep strain (G1) is dominated in human patients in Great Khorasan, the prevention of camel-dog cycle should pay attention in this region.