Open AccessIsolation and immunogenicity of extracted outer membrane vesicles from Pseudomonas aeruginosa under antibiotics treatment conditions
Author(s) -
Mehdi HadadiFishani,
Shahin Najar Peerayeh,
Seyed Davar Siadat,
Mohammad Hadi Sekhavati,
Ashraf Mohabati Mobarez
Publication year - 2021
Publication title -
iranian journal of microbiology.
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.37
H-Index - 27
eISSN - 2008-4447
pISSN - 2008-3289
DOI - 10.18502/ijm.v13i6.8087
Subject(s) - amikacin , microbiology and biotechnology , pseudomonas aeruginosa , bacterial outer membrane , immune system , antibiotics , immunogenicity , antibody , meropenem , secretion , chemistry , biology , immunology , bacteria , antibiotic resistance , biochemistry , escherichia coli , genetics , gene
Background and Objectives: Different types of antibiotics have been indicated to enhance the secretion of OMVs from Pseudomonas aeruginosa. We aimed to investigate the effect of meropenem and amikacin antibiotics on inducing the secre- tion of OMVs and immunologic features in P. aeruginosa.
Materials and Methods: The OMVs were prepared from P. aeruginosa under hypervesiculation condition (treatment with amikacin and meropenem), and extraction was carried out by the sequential ultracentrifugation. Physicochemical features of extracted OMVs were evaluated by electron microscopy and SDS-PAGE. To quantify antibody synthesis and function after immunization with OMV, we used ELISA, serum bactericidal activity, and opsonophagocytosis. Production of cytokines from splenocytes of immunized mice was measured with ELISA.
Results: Specific-antibody IgG production, particularly IgG1 subclass, increased in mice primed with hypervesiculation-de- rived OMVs compared to normal condition-derived OMVs. Serum bactericidal activity and opsonophagocytosis of secreted antibody was enhanced in mice primed with hypervesiculation-derived OMVs. Investigation of cytokine production showed the upregulation of IL-8, IL-12, IL-17, and TNF-α and downregulation of IL-10.
Conclusion: Based on our findings, OMVs production can be increased by treating P. aeruginosa with amikacin and mero- penem antibiotics. Moreover, hypervesiculation-derived OMV scan possibly activate the humoral and cellular immune re- sponse more than normal OMVs.