Open AccessA comparative characterization of nasal and clinical isolates of Staphylococcus aureus from west of Iran
Author(s) -
Gholamreza Goudarzi,
Yaser Hasanvand,
Fahimeh Rezaei,
Somayeh Delfani
Publication year - 2021
Publication title -
iranian journal of microbiology.
Language(s) - English
Resource type - Journals
eISSN - 2008-4447
pISSN - 2008-3289
DOI - 10.18502/ijm.v13i6.8086
Subject(s) - staphylococcus aureus , anterior nares , microbiology and biotechnology , sccmec , antibiotics , medicine , antibiotic resistance , enterotoxin , clindamycin , methicillin resistant staphylococcus aureus , biology , gene , bacteria , biochemistry , genetics , escherichia coli
Background and Objectives: Recently, the rise of methicillin-resistant Staphylococcus aureus (MRSA) isolated from hos- pital healthcare workers (HCWs) and various infectious samples has become one of the main concerns in hospital settings. Therefore, epidemiological studies are necessary to monitor antibiotic resistance patterns in each region and to study the pathogenesis of this strain to control infections.
Materials and Methods: In this cross-sectional study, a total of 100 S. aureus isolates, including 50 isolates obtained from the anterior nares of healthcare workers, as well as 50 other isolates cultured from the various clinical specimens from the referral hospitals in Khorramabad (West of Iran) were tested. All isolates were examined to determine antibiotic resistance pattern, and the presence of staphylococcal enterotoxin A (sea), staphylococcal enterotoxin B (seb) and mecA genes.
Results: The mecA gene was found among 36% (18/50) of the clinical S. aureus isolates (CSIs) and 14% (7/50) of nasal S. aureus isolates (NSIs), with statistically significant difference (X2 = 6.53; p = 0.011). The difference between the frequency rate of sea gene among MRSA strains isolated from clinical specimens (46.6%, 7/15) was significant compared to strains isolated from nostrils (14.3%, 1/7) (X2 = 3.85; p = 0.049).
Conclusion: The frequency of mecA, sea, and seb genes among the clinical samples was more than strains isolated from the nostrils of healthcare personnel.