Open AccessMolecular characterization of healthcare and community-associated methicillin-resistant Staphylococcus aureus using phage open-reading frame typing
Author(s) -
Takaaki Konuma,
Shunsuke Takahashi,
Masato Suzuki,
Arinobu Tojo
Publication year - 2021
Publication title -
iranian journal of microbiology.
Language(s) - English
Resource type - Journals
eISSN - 2008-4447
pISSN - 2008-3289
DOI - 10.18502/ijm.v13i4.6985
Subject(s) - multilocus sequence typing , staphylococcus aureus , virulence , microbiology and biotechnology , typing , methicillin resistant staphylococcus aureus , antibiotic resistance , biology , phage typing , antibiotics , gene , genetics , genotype , bacteria
The polymerase chain reaction-based open reading frame typing (POT) method is a simple and rapid method for the strain-level discrimination of methicillin-resistant Staphylococcus aureus (MRSA). We investigated the molecular charac- teristics of S. aureus strains by multilocus sequencing typing (MLST) and POT and the profiles of antibiotic resistance and virulence genes of MRSA isolates in a single center of Tokyo, Japan. Five types by MLST and 19 types by POT were detected in the 25 MRSA isolates. ST5 and a POT1 score of 93 were associated with healthcare-associated MRSA, whereas ST8 and a POT1 score of 106 were associated with community-associated MRSA. Each strain evaluated by POT score was completely associated with similar profiles of antibiotic resistance and virulence genes. These data showed that the POT system was a powerful molecular tool for the epidemiological characterization of MRSA isolates, which correlated with the profiles of antibiotic resistance and virulence genes.