Open AccessExpression and Characterization of Two DNA Constructs Derived from HIV-1-vif in Escherichia coli and Mammalian Cells
Author(s) -
Fatemeh Zamani,
Azam Bolhassani,
Sepideh Shahbazi,
Ahmad Faghih,
Seyed Mehdi Sadat
Publication year - 2021
Publication title -
avicenna journal of medical biotechnology.
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.399
H-Index - 22
eISSN - 2008-4625
pISSN - 2008-2835
DOI - 10.18502/ajmb.v13i3.6369
Subject(s) - transfection , plasmid , biology , hek 293 cells , microbiology and biotechnology , recombinant dna , gene , blot , flow cytometry , green fluorescent protein , virology , antibody , genetics
Acquired immunodeficiency syndrome (HIV/AIDS) is still a major global concern and no effective therapeutic vaccine has been produced to prevent the problem. Among HIV-1 proteins, vif as a basic cytoplasmic protein of HIV-1 is involved in late stages of viral generation and plays important role in HIV-1 virion replication. It also increases the stability of virion cores, which probably inhibits early degradation of viral entry. Therefore, it seems rational to apply this protein as a vaccine based on its impact on HIV-1 life cycle. This study aimed at cloning, expression and production of vif protein as an HIV-1 vaccine candidate.