Open AccessDetection of Aneuploidies in Products of Conception and Neonatal Deaths in Iranian Patients Using the Multiplex Ligation-Dependent Probe Amplification (MLPA)
Author(s) -
Sara Khorami Sarvestani,
Maryam Rafati,
Haleh Soltanghoraee,
Azadeh Hoseini,
Azadeh Soltani,
Koosha Jalilian,
Saeed Reza Ghaffari
Publication year - 2021
Publication title -
avicenna journal of medical biotechnology.
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.399
H-Index - 22
eISSN - 2008-4625
pISSN - 2008-2835
DOI - 10.18502/ajmb.v13i3.6363
Subject(s) - multiplex ligation dependent probe amplification , trisomy , multiplex , aneuploidy , obstetrics , karyotype , prenatal diagnosis , products of conception , biology , monosomy , fetus , chromosome , gynecology , medicine , genetics , pregnancy , abortion , gene , exon
Around 70% of all pregnancies (Including 15% of clinically-recognized ones) are lost due to various fetal or maternal disorders. Chromosomal aneuploidies are among the most common causes of pregnancy loss. Standard chromosome analysis using G-banding technique (Karyotype) is the technique of choice in studying such abnormalities; however, this technique is time-consuming and sensitive, and limited by vulnerabilities such as cell culture failure. The use of molecular cytogenetic techniques, including array-based techniques and Multiplex Ligation-Dependent Probe Amplification (MLPA), has been proposed to overcome the limitations of this method to study the products of conception. This study has been designed to investigate the feasibility of using MLPA technique as a standalone genetic testing, with histopathologic examinations and genetic counseling to detect aneuploidies in products of conception and neonatal deaths.