Open AccessMolecular Epidemiology of blaCMY-1, blaCMY-2, blaFOX Genes in K. pneumoniae From Elderly Patients in Tehran, Iran
Author(s) -
Ali Badamchi,
Alireza Namazi Shabestari,
Mohammad Etemadi,
Azardokht Tabatabaei
Publication year - 2021
Publication title -
acta medica iranica.
Language(s) - English
Resource type - Journals
eISSN - 1735-9694
pISSN - 0044-6025
DOI - 10.18502/acta.v59i8.7250
Subject(s) - cefoxitin , klebsiella pneumoniae , cephalosporin , medicine , antibiotics , microbiology and biotechnology , tazobactam , carbapenem , piperacillin/tazobactam , piperacillin , antibiotic resistance , population , gene , bacteria , biology , escherichia coli , imipenem , genetics , pseudomonas aeruginosa , environmental health , staphylococcus aureus
The growth rate of the population aging is increasing worldwide. To assess antimicrobial susceptibility of extended-spectrum β-lactamase- (ESBL-) producing Klebsiella pneumoniae Isolated from Patients aging in Rasool Akram, Hospital, as well as to identify AmpC genes. 100 K. pneumoniae strain isolated from different clinical samples. Isolates resistant to oxyimino-cephalosporins and to cefoxitin evaluated to phenotypic ESBL production and to phenotypic AmpC production, respectively. Detection of resistance genes was then performed using primers specific for AmpC genes. Piperacillin/tazobactam and carbapenems remained the active β-lactam antibiotic against K. pneumoniae. ESBLs were detected among 40 (40%) of K. pneumoniae isolates. CMY-1 gene was detected in 34.3% of all AmpC-positive isolates, whereas CMY-2 and FOX genes were 14.2% and 28.6%, respectively. The consumption of Carbapenem family drugs is high in Iranian hospitals which are used as a first line of treatment without antibiotic susceptibility testing. Therefore, increase in antibiotic resistance to this family drugs is unavoidable in the near future. Therefore, it is necessary to take the necessary measures to modify the administration and use of antibiotics.