Open AccessAnalysis of phagocytic activity of macrophages of monocytic origin and Kupfer cells
Author(s) -
Andrey Elchaninov,
Anastasia Lokhonina,
А. В. Макаров,
И В Арутюнян,
M V Grinberg,
Г. А. Ладыгина,
Л. А. Князева,
Galina Bolshakova,
Timur Fatkhudinov
Publication year - 2018
Publication title -
žurnal anatomii i gistopatologii
Language(s) - English
Resource type - Journals
ISSN - 2225-7357
DOI - 10.18499/2225-7357-2018-7-3-13-19
Subject(s) - macrophage , cd68 , mononuclear phagocyte system , macrophage activating factor , phenotype , kupffer cell , phagocytosis , monocyte , biology , microbiology and biotechnology , thp1 cell line , mannose receptor , tumor necrosis factor alpha , cell culture , chemistry , immunology , in vitro , immunohistochemistry , biochemistry , genetics , gene
The purpose of the study was to compare the phagocytic activity of macrophages of monocytic origin both without activation and under the influence of factors of the M1 and M2 phenotype. Material and methods. Peripheral blood monocytes and Kupper liver cells of male Wistar rats were obtained by gradient centrifugation. The Kupffer cells and rat monocytes were transferred to RPMI growth medium. To activate in the direction of the M1-phenotype, LPS and IFN-γ were introduced into the medium. To activate the M2 phenotype, IL 4, IL10, and IL 13 were added to the medium. The obtained macrophage cultures were stained with antibodies to CD68. To study the phagocytic activity of macrophages, the cells were placed on plates for intravital microscopy and latex beads were added to the culture medium. Results. The macrophage cultures of monocytic origin and Kupffer cells expressed CD68 at a high level, the addition of activation factors did not change the expression of the marker. 1 hour after the addition of latex particles to the culture medium, unactivated monocytic macrophages statistically significantly absorbed particles more than Kupffer cells. Activation by factors of the M1 and M2 phenotype led to an increase in the phagocytic activity of both macrophages of monocytic origin and Kupffer cells. The most activating effect on phagocytic activity was provided by induction factors of the M1 phenotype. Conclusions. For macrophages of monocytic origin and Kupffer cells, a different dynamics of phagocytic activity is characteristic. Monocytic macrophages initially have a more pronounced absorption capacity, which gradually increases during the experiment. For Kupffer cells, a sharp fluctuation of phagocytic activity is characteristic: rapid growth and rapid saturation.