Open AccessFunctional and Proliferative Activity of Cells of Neuro-Glio-Vascular Complexes of Sensorimotor Cortex, Hippocampus and Amygdala of White Rats Brain During Early Postischemic Period
Author(s) -
А.С. Степанов,
В. А. Акулинин,
Д. Б. Авдеев,
А. В. Горбунова
Publication year - 2018
Publication title -
žurnal anatomii i gistopatologii
Language(s) - English
Resource type - Journals
ISSN - 2225-7357
DOI - 10.18499/2225-7357-2018-7-2-85-89
Subject(s) - microglia , hippocampus , cortex (anatomy) , neuroglia , white matter , immunohistochemistry , cerebral cortex , pathology , astrocyte , medicine , neuroscience , biology , anatomy , central nervous system , inflammation , magnetic resonance imaging , radiology
The aim of the study is to determine functional and proliferative activity of sensorimotor cortex (SMC), hippocampus and amygdala (AM) cells of the brain of white rats in normal and after 20-minute occlusion of the common carotid arteries (without hypotension). Material and methods. Using light microscopy and immunohistochemistry were studied neurons, astrocytes, oligodendroglia and microglia brain normal (n=5) and after 6 h, 1, 3 days (n=15) after acute ischemia. Antibodies to glial fibrillar acid protein (GFAP) and Ki67 (manufacturer: Leica Biosystems Newcastle Ltd, UK) were used. Results. After reperfusion, against the background of neuronal damage (swelling, dystrophic and necrobiotic changes), processes of neuroglia cell proliferation were activated, expression of GFAP (hypertrophy of astrocytes) increased, neuroglial index increased, a large number of satellite astrocytes appeared around large neurons of the SMC. After 3 days in the SMC the content of Ki67-positive cells increased to 18.6% (95% CI: 13.5-24.7%) of all gliocytes, in the hippocampus and AM - respectively - to 7.5% (95% CI: 4.3-12.1%) and 10.7% (95% CI: 6.8-15.9%). Thus, statistically significant differences were revealed between the compared parts of the brain. Conclusion. In the acute period after a 20-minute occlusion of the common carotid arteries in the SMC, the hippocampus and the AM increased functional and proliferative activity of astrocytes, oligodendroglia and microglia, which functioned as a single integrated protective and insulating cellular system with a strong regional specificity.