Open AccessBone Subdestructive Kryoapplicaton in the Experiment
Author(s) -
Ivan Ivanovich Kuzhelivskiy,
Grigoriy Vladimivorich Slizovskiy,
Леонид Александрович Ситко
Publication year - 2017
Publication title -
vestnik èksperimentalʹnoj i kliničeskoj hirurgii
Language(s) - English
Resource type - Journals
eISSN - 2409-143X
pISSN - 2070-478X
DOI - 10.18499/2070-478x-2017-10-1-44-48
Subject(s) - cryotherapy , calcification , refrigerant , animal study , biomedical engineering , bone tissue , medicine , surgery , pathology , mechanical engineering , engineering , gas compressor
Relevance. Trend of modern surgery in the implementation of new methods of treatment is to develop all phases of the proposed method of treatment in an experimental model. In this paper, comparative studies have been performed in laboratory animals, aimed at studying the bone tissue reaction at subdestruktion cryotherapy.
Purpose. Identify the optimal exposure of the refrigerant to achieve kryoregenerative action ultralow temperatures.
Materials and methods. An experimental study was conducted on rabbits "Chinchilla" by cryotherapy in the cavity osteoperforation with different exposure refrigerant (liquid nitrogen). After 10 days after the removal of the animal from an experiment conducted morphological study.
Results. It was found that the exposure of 3 seconds is observed active regeneration, while kryoosteonekrosis observed during long-term exposure. The experimental results allow differentiated recommend them for use in clinical practice.
Conclusion. Light microscopy kryoregeneration with a three-second exposure shows the refrigerant is very active calcification regenerate. Primary callus composed of fibrous tissue and retikulofibrosis. Reclaimed presented osteoblasts chain. The proposed regime is advisable to apply cryotherapy with degenerative diseases of the bone in order to increase osteoreparative properties of bone.