Open AccessUse of transcriptional slippage for diverse gene expression
Author(s) -
Dawid Koscielniak,
Ewelina Sobisz,
Ewa Wons,
Marian Sęktas
Publication year - 2021
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.2020_5736
Subject(s) - slippage , gene , genetics , t7 rna polymerase , biology , gene expression , open reading frame , promoter , mutation , rna polymerase , computational biology , rna , structural engineering , escherichia coli , bacteriophage , engineering , peptide sequence
We present here an alternative for two-promoter systems ensuring highly diverse expression of several genes from a single promoter. This approach assumes an introduction of a deletion mutation into an A/T homopolymeric run in a gene’s proximal part, and employs the transcriptional slippage mechanism for insertion-dependent reinstatement of the proper reading frame by the T7 RNA polymerase.