Open AccessA rapid method for protein staining in polyacrylamide gels using water saturated with chloroform
Author(s) -
P. Pięta,
Ewa Burchacka,
Aleksandra Śliwa,
Anna Szczerba
Publication year - 2021
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.2020_5678
Subject(s) - staining , polyacrylamide , polyacrylamide gel electrophoresis , chloroform , chromatography , chemistry , coomassie brilliant blue , silver stain , fluorescence , microbiology and biotechnology , biochemistry , biology , polymer chemistry , enzyme , genetics , physics , quantum mechanics
Polyacrylamide gel electrophoresis, followed by an appropriate staining, is a popular and useful analytical procedure for protein identification and characterization. The aim of this study was to develop a method for protein visualization in polyacrylamide gels that would be alternative to Coomassie blue or silver staining. The proposed method is simple, fast and inexpensive. The optimized protocol for protein staining and visualization takes as little as 6 minutes and utilizes deionized water and chloroform. Fluorescence of proteins is induced by UV light and can be detected with a standard transilluminator.