Open AccessThe effect of miR-496 on eIF3h in lung invasive adenocarcinoma
Author(s) -
Ji-Chun
Publication year - 2021
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.2020_5491
Subject(s) - adenocarcinoma , immunohistochemistry , stage (stratigraphy) , lung , pathology , medicine , microrna , lymph node metastasis , real time polymerase chain reaction , metastasis , biology , cancer , gene , paleontology , biochemistry
This study explores the correlation between microRNA-496 (miR-496) expression and eukaryotic translation initiation factor 3H (eIF3h) expression in lung invasive adenocarcinoma. A total of 71 patients with lung adenocarcinoma were included in the study. The tumor tissue samples were collected together with the normal lung tissue samples that were acquired more than 2 cm away from the edge of the tumor). Expression of miR-496 was detected using real-time fluorescence quantitative PCR. Expression of Ki67 and Bcl-2 in lung adenocarcinoma was detected with Western blotting, and expression of eIF3hwas detected with immunohistochemistry. Expression of miR-496 was significantly lower in the tumor than in normal lung tissue (P 0.05). Low expression of miR-496 correlated with a shorter survival time of the patients (P<0.05). Moreover, miR-496 expression was negatively correlated with Ki67, Bcl-2 and eIF3h expression. We speculate that and miR-496 has the potential to regulates eIF3h expression, and low expression of miR-496 in lung adenocarcinoma may be related to the occurrence and development of the tumor. Mir-496 may be an independent prognostic factor in lung adenocarcinoma.