Open AccessResveratrol enhances apoptosis induced by the heterocyclic aromatic amines in p53-wt LoVo cells, but not in p53-deficient HaCaT cells
Author(s) -
Katarzyna Kuryłowicz,
Agnieszka Cierniak,
Ewelina Madej,
Łukasz Skalniak,
Agnieszka Wolnicka-Głubisz
Publication year - 2020
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.2020_5477
Subject(s) - hacat , apoptosis , resveratrol , chemistry , dna damage , cancer research , microbiology and biotechnology , cell culture , cell , cancer cell , dna , in vitro , biochemistry , cancer , biology , genetics
In the present study, we investigated the influence of resveratrol on PhIP treated human colon cancer cells and compared the effect to HaCaT cells considered as normal, human keratinocytes. Our results show that resveratrol decreases DNA damage in both cell types, it increases the sensitivity of LoVo cells to apoptosis and has no effect on PhIP-treated HaCaT cells. We confirm that PhIP-induced apoptosis is p53 and caspase 3/7 dependent. Interestingly, normal cells such as HaCaT, which lack functional p53 are more resistant to PhIP treatment.