Open AccessCladonia uncialis as a valuable raw material of biosynthetic compounds against clinical strains of bacteria and fungi
Author(s) -
Elżbieta Studzińska-Sroka,
Hanna Tomczak,
Natalia Malińska,
Marta Wrońska,
Robert Kleszcz,
Agnieszka Galanty,
Judyta CieleckaPiontek,
Dorota Latek,
Jarosław Paluszczak
Publication year - 2019
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.2019_2891
Subject(s) - gallic acid , usnic acid , hacat , enterococcus faecium , chemistry , dpph , antibacterial activity , caffeic acid , antioxidant , bacteria , microbiology and biotechnology , food science , biology , biochemistry , botany , antibiotics , lichen , in vitro , genetics
Cladonia uncialis is a lichen species with confirmed antibacterial activity and whose genome has been recently sequenced, enabling first attempts in its functional characterization. In this work, we investigated activity of the C. uncialis acetone extract (CUE) and usnic acid (UA) enantiomers against ten clinical microbial strains causing skin infections. The results showed that CUE, containing (–)-UA and squamatic acid, assayed at the same concentrations as UA, was noticeably more active than (–)-UA alone, in its pure form. The studied CUE displayed an activity that was comparable to that of (+)-UA observed for Staphylococcus epidermidis and Enterococcus faecium (18-24 mm zone of growth inhibition), but did not display any activity against fungal strains. The CUE demonstrated low cytotoxicity against HaCaT cells, in comparison to UA enantiomers, which is important for its therapeutic use. Results of the antioxidant assay (DPPH) indicated low antioxidant activity (IC50>200 µg/mL) of CUE, while the total phenolic content was 70.36 mg Gallic Acid Equivalent/g of the dry extract.