Open AccessLack of association between UBE2E2 gene polymorphism (rs7612463) and type 2 diabetes mellitus in a Saudi population.
Author(s) -
Khalid Khalaf Alharbi,
Imran Ali Khan,
Yazeed A. AlSheikh,
Fawiziah Khalaf Alharbi,
Fahad Khalaf Alharbi,
May Al-Nbaheen
Publication year - 2014
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.2014_1844
Subject(s) - type 2 diabetes mellitus , genotype , medicine , allele , polymorphism (computer science) , diabetes mellitus , type 2 diabetes , anthropometry , population , endocrinology , gene , biology , genetics , environmental health
The ubiquitin-conjugating enzyme E2E 2 (UBE2E2) gene plays an important role in insulin synthe-sis and secretion under conditions in which stress to the endoplasmic reticu-lum is increased in β-cells. In this case-control study, we have selected rs7612462 polymorphism within UBE2E2 gene to identify in a Saudi population the type 2 diabetes mellitus (T2DM) subjects. In total, 376 subjects with T2DM and 380 controls were enrolled in this study. We have collected 5 mL of peripheral blood from each participant for biochemical and molecular analyses. PCR-RFLP was used to generate genotypes at rs7612462 in all of the study subjects. Clinical data and anthropometric measurements of the patients were significantly different from those of the controls (p