Open AccessRibozymes of the hepatitis delta virus: recent findings on their structure, mechanism of catalysis and possible applications.
Author(s) -
Jerzy Ciesiołka,
Jan Wrzesiński,
Michał Łęgiewicz,
Barbara Smólska,
Mariola Dutkiewicz
Publication year - 2001
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.2001_3925
Subject(s) - ribozyme , ligase ribozyme , hairpin ribozyme , rna , chemistry , nucleotide , nucleic acid structure , mammalian cpeb3 ribozyme , catalysis , hepatitis delta , mechanism (biology) , computational biology , molecule , combinatorial chemistry , stereochemistry , biology , biochemistry , physics , gene , organic chemistry , quantum mechanics
Although the delta ribozymes have been studied for more than ten years the most important information concerning their structure and mechanism of catalysis were only obtained very recently. The crystal structure of the genomic delta ribozyme turns out to be an excellent example of the extraordinary properties of RNA molecules to fold into uniquely compact structures. Details of the X-ray structure have greatly stimulated further studies on the folding of the ribozymes into functionally active molecules as well as on the mechanism of RNA catalysis. The ability of the delta ribozymes to carry out general acid-base catalysis by nucleotide side chains has been assumed in two proposed mechanisms of self-cleavage. Recently, considerable progress has been also made in characterizing the catalytic properties of trans-acting ribozyme variants that are potentially attractive tools in the strategy of directed RNA degradation.