Open AccessThe carbohydrate moiety of human glycophorin in CDG syndrome.
Author(s) -
Bożena Krotkiewska,
Krzysztof Zwierz,
Hubert Krotkiewski
Publication year - 1999
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1999_4170
Subject(s) - glycophorin , sialoglycoprotein , glycosylation , glycan , glycoprotein , biochemistry , carbohydrate , chemistry , glycobiology , sialoglycoproteins , biology , membrane
Human glycophorin, the major sialoglycoprotein of erythrocyte membranes, was isolated from erythrocytes of healthy individuals and four patients with CDG syndrome. Sugar analysis revealed lower carbohydrate content in three out of four CDG-glycophorin samples. In order to characterize closer the glycosylation differences between glycophorin samples in health and disease, reaction with four biotinylated lectins was performed, using ELISA procedure on polystyrene microplates. Results obtained so far strongly suggest that both N- and O-glycans of glycophorin are affected in CDG syndrome.