Open AccessThe essential DNA polymerases delta and epsilon are involved in repair of UV-damaged DNA in the yeast Saccharomyces cerevisiae.
Author(s) -
Agnieszka Hałas,
Zofia Policińska,
Hanna Maria Baranowska,
Witold J. Jachymczyk
Publication year - 1999
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1999_4162
Subject(s) - dna polymerase , dna polymerase ii , dna repair , biology , dna polymerase delta , dna polymerase mu , dna , dna clamp , dna replication , polymerase , saccharomyces cerevisiae , microbiology and biotechnology , dna polymerase i , nucleotide excision repair , genetics , yeast , gene , circular bacterial chromosome , polymerase chain reaction , reverse transcriptase
We have studied the ability of yeast DNA polymerases to carry out repair of lesions caused by UV irradiation in Saccharomyces cerevisiae. By the analysis of postirradiation relative molecular mass changes in cellular DNA of different DNA polymerases mutant strains, it was established that mutations in DNA polymerases delta and epsilon showed accumulation of single-strand breaks indicating defective repair. Mutations in other DNA polymerase genes exhibited no defects in DNA repair. Thus, the data obtained suggest that DNA polymerases delta and epsilon are both necessary for DNA replication and for repair of lesions caused by UV irradiation. The results are discussed in the light of current concepts concerning the specificity of DNA polymerases in DNA repair.