Open AccessCharacterization of DNA sequences localized 11 to 17.5 kb upstream of the chicken embryonic pi-globin gene.
Author(s) -
Monika Pietrowska,
Marek Rusin,
Piotr Widłak,
S. V. Razin,
Joanna Rzeszowska-Wolny
Publication year - 1999
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1999_4150
Subject(s) - microbiology and biotechnology , gene , biology , dna , globin , transfection , gene cluster , genetics
We have analyzed the DNA fragment localized about 11 to 17.5 kb upstream of the chicken alpha-globin gene domain (the fragment was designed as alpha-0). The nucleotide sequence of its 3.3 kb-long 5' part was established and interactions with nuclear matrix proteins were studied. The DNA region localized about 16 kb upstream of the embryonic pi-globin gene showed high affinity to nuclear matrices in vitro. Two palindromes and a cluster of inverted repeats were co-localized in the same region. The whole 6.6 kb alpha-0 fragment decreased the activity of linked CAT reporter gene when transfected into chicken erythroblastoid cells.