Application of aqueous hydrazine solution for beta-elimination of O-glycans from gastric mucin. | Zendy

Dariusz G. Kisiel | Zendy; A Gindzieński | Zendy; Adam Gądek | Zendy

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Application of aqueous hydrazine solution for beta-elimination of O-glycans from gastric mucin.

Author(s) -

Dariusz G. Kisiel,

A Gindzieński,

Adam Gądek

Publication year - 1999

Publication title -

acta biochimica polonica

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.452

H-Index - 78

eISSN - 1734-154X

pISSN - 0001-527X

DOI - 10.18388/abp.1999_4147

Subject(s) - chemistry , hydrazine (antidepressant) , glycan , aqueous solution , mucin , chromatography , dextran , triethylamine , derivatization , organic chemistry , biochemistry , glycoprotein , high performance liquid chromatography

O-Glycans from pig gastric mucin were released by beta-elimination in 0.2 M triethylamine and 50% aqueous hydrazine solution. The released glycan hydrazides were isolated using Centricon 10 separators, brought to their reducing form and reductive by labelled with p-aminobenzoic acid ethyl ester (ABEE). Labelled products were fractionated into neutral and acid fractions on a Bio-Gel P4 column, calibrated with a mixture of dextran oligosaccharides, labelled according to the same procedure.

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