Open AccessApplication of aqueous hydrazine solution for beta-elimination of O-glycans from gastric mucin.
Author(s) -
Dariusz G. Kisiel,
A Gindzieński,
A Gadek
Publication year - 1999
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1999_4147
Subject(s) - chemistry , hydrazine (antidepressant) , glycan , aqueous solution , mucin , chromatography , dextran , triethylamine , derivatization , organic chemistry , biochemistry , glycoprotein , high performance liquid chromatography
O-Glycans from pig gastric mucin were released by beta-elimination in 0.2 M triethylamine and 50% aqueous hydrazine solution. The released glycan hydrazides were isolated using Centricon 10 separators, brought to their reducing form and reductive by labelled with p-aminobenzoic acid ethyl ester (ABEE). Labelled products were fractionated into neutral and acid fractions on a Bio-Gel P4 column, calibrated with a mixture of dextran oligosaccharides, labelled according to the same procedure.