Open AccessProtection of cattle against bovine leukemia virus (BLV) infection could be attained by DNA vaccination.
Author(s) -
A Brillowska,
Sławomir Dąbrowski,
J. Rułka,
P. Kubis,
E. Buzała,
Józef Kur
Publication year - 1999
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1999_4121
Subject(s) - bovine leukemia virus , virology , biology , dna vaccination , plasmid , immune system , virus , glycoprotein , vector (molecular biology) , gene , antibody , vaccination , recombinant dna , microbiology and biotechnology , immunology , genetics
The bovine leukemia virus (BLV) envelope gene encoding extracellular glycoprotein gp51 and transmembrane glycoprotein gp30 was cloned into a vehicle expression vector under the human cytomegalovirus (CMV) intermediate early promoter. The intramuscular injection of this plasmid vector generated a cellular immune response. Seven out of ten cows vaccinated with the DNA construct resisted a drastic challenge (500 BLV-infected lymphocytes as an infectious dose).