Open AccessFunctional role of rRNAs in plant translation system tested with antisense strategy.
Author(s) -
M Nawrot,
Ewa Bohun,
Lilla Książczak-Kus,
Andrzej Sobkiewicz,
Joseph F. Polak,
Tomasz Twardowski
Publication year - 1998
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1998_4363
Subject(s) - ribosomal rna , ribosome , translation (biology) , biology , protein biosynthesis , 23s ribosomal rna , 30s , protein secondary structure , peptidyl transferase , biochemistry , genetics , rna , computational biology , messenger rna , gene
There are regions in rRNA which are evolutionary conserved and exposed on ribosomal surface. We selected in plant material (Lupinus luteus) two of them: the alpha-sarcin domain of 26S rRNA (L-rRNA) and C loop of 5S rRNA, to be further investigated using antisense oligomers as research tools. We found inhibition of the model polypeptide biosynthesis (up to 80%) due to specific hybridization of oligomers addressed to alpha-sarcin domain and loop C. Based on our results we present the evidence for the key role played by these regions of rRNAs during protein biosynthesis in plant system. According to our hypothesis, conformational changes of these two regions are synchronised and cooperative during transition of pre- to post-translocation state of the ribosome. The correlation of structure and activity of rRNA domains in translation is shown.