Open AccessSynthesis, biochemical and biological studies on oligonucleotides bearing a lipophilic dimethoxytrityl group.
Author(s) -
Konrad Misiura,
Marzena Wójcik,
Agnieszka Krakowiak,
Maria Koziołkiewicz,
Zofia Pawłowska,
Elżbieta Pluskota,
Czesław S. Cierniewski,
Wojciech J. Stec
Publication year - 1998
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1998_4315
Subject(s) - oligonucleotide , cleavage (geology) , chemistry , rnase p , conjugate , rnase h , biochemistry , plasminogen activator , microbiology and biotechnology , biology , rna , dna , gene , genetics , paleontology , mathematical analysis , mathematics , fracture (geology)
Dimethoxytritylphosphono-oligonucleotide conjugates have been prepared. They are totally resistant to nucleases present in human serum and do not affect cleavage of a complementary oligoribonucleotide by RNase H. Conjugates possessing a phosphate backbone gave better antisense inhibition of expression of plasminogen activator inhibitor type-1 within endothelial cells as compared with unconjugated oligonucleotides.