Open AccessEffect of DNA-interacting drugs on phage T7 RNA polymerase.
Author(s) -
Mariola K. Piestrzeniewicz,
K Studzian,
D Wilmańska,
G Płucienniczak,
M Gniazdowski
Publication year - 1998
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1998_4295
Subject(s) - dna , polymerase , rna , chemistry , rna polymerase , acridine orange , rna dependent rna polymerase , dna polymerase , microbiology and biotechnology , biochemistry , biology , gene , apoptosis
9-Aminoacridine carboxamide derivatives studied here form with DNA intercalative complexes which differ in the kinetics of dissociation. Inhibition of total RNA synthesis catalyzed by phage T7 and Escherichia coli DNA-dependent RNA polymerases correlates with the formation of slowly dissociating acridine-DNA complex of time constant of 0.4-2.3 s. Their effect on RNA synthesis is compared with other ligands which form with DNA stable complexes of different steric properties. T7 RNA polymerase is more sensitive to distamycin A and netropsin than the E. coli enzyme while less sensitive to actinomycin D. Actinomycin induces terminations in the transcript synthesized by T7 RNA polymerase. Despite low dissociation rates of DNA complexes with acridines and pyrrole antibiotics no drug dependent terminations are observed with these ligands.