Open AccessReconstitution of UV-damaged DNA into chromatin using Xenopus oocyte extracts.
Author(s) -
Piotr Widłak
Publication year - 1998
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1998_4252
Subject(s) - chromatin , xenopus , oocyte , dna , nucleosome , microbiology and biotechnology , biology , plasmid , dna damage , chemistry , genetics , embryo , gene
Chromatin was reconstituted in vitro using Xenopus oocyte extracts and plasmid DNA containing UV radiation-induced damage. Damaged DNA was assembled into minichromosomes with an efficiency similar to that of control, non-irradiated DNA. Oocyte extracts were competent to carry out DNA repair, which was elicited by nicking damaged templates followed by DNA synthesis during chromatin assembly. Newly synthesized DNA was efficiently reconstituted into nucleosomes.