Open AccessStudies on the translation mechanism of subgenomic RNA of potato leafroll virus.
Author(s) -
Marek Juszczuk,
Włodzimierz Zagórski-Ostoja,
D Hulanicka
Publication year - 1997
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1997_4441
Subject(s) - subgenomic mrna , open reading frame , orfs , biology , start codon , translation (biology) , eukaryotic translation , rna , five prime untranslated region , gene , stop codon , microbiology and biotechnology , untranslated region , virology , genetics , messenger rna , peptide sequence
The expression of open reading frames located on the subgenomic RNA (sgRNA) has been studied in an in vitro transcription and translation system. The obtained results indicate: a) translation of sgRNA occurs according to the scanning model since the insertion of palindrome (delta G0 = -61 kcal/mol) prevents the initiation of translation; b) ORF6 is translated by suppression of the stop codon separating ORF4 from ORF6 and the presence of suppressor tRNA is necessary for the readthrough; c) the presence of leader sequence of sgRNA (212 nucleotides) decreases the translation efficiency of ORFs located downstream and it affects the ratio of products of ORF4 and ORF5; d) 3'UTR does not influence on an expression of genes located on the sgRNA.