Open AccessStudies on the nature of genotoxic and cytotoxic effects induced by hydralazine.
Author(s) -
J Marczewska,
J Koziorowska
Publication year - 1997
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1997_4426
Subject(s) - genotoxicity , superoxide dismutase , catalase , cytotoxicity , chemistry , dimethyl sulfoxide , cytotoxic t cell , hydralazine , pharmacology , biochemistry , in vitro , microbiology and biotechnology , toxicity , enzyme , biology , endocrinology , organic chemistry , blood pressure
The nature of genotoxic and cytotoxic effects induced by hydralazine was analyzed taking into account possible protection of cells by catalase, superoxide dismutase and dimethyl sulfoxide. For the experiments designed to evaluate the influence of scavengers on the genotoxicity expressed as the SOS induction factor the E. coli PQ37 strain was used. The cytotoxic effects were investigated in V3 cells cultured in vitro. The genotoxicity and cytotoxicity of hydralazine were suppressed by catalase in a dose-dependent manner but they were enhanced by superoxide dismutase. No protective effect of dimethyl sulfoxide was observed. Our results indicate that H2O2 plays an essential role in the genotoxicity and cytotoxicity of hydralazine.