Open AccessImmunochemical characterization of lipopolysaccharide from glucose-nonfermenting gram-negative clinical bacterial isolate.
Author(s) -
Małgorzata Mieszała,
J Kübler,
Andrzej Gamian
Publication year - 1997
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1997_4424
Subject(s) - microbiology and biotechnology , lipopolysaccharide , heptose , bacteria , chemistry , gram negative bacteria , strain (injury) , fatty acid , biochemistry , lipid a , bacterial outer membrane , biology , escherichia coli , genetics , anatomy , mutant , gene , endocrinology
A glucose-nonfermenting Gram-negative bacterial strain isolated from bronchofiberoscope used for examination of the patients suffering from pulmonary diseases was subjected to phenol-water extraction. Lipopolysaccharides (LPS) isolated from the water and the phenol phase differed in fatty acid composition. Both contained xylose, glucose, glucosamine and components typical for LPS, namely heptose, 3-deoxyoctulosonic acid (Kdo) and 3-hydroxymyristic acid. The presence of sphingosine in all LPS preparations classifies the strain to the genus Sphingomonas.