Open AccessCharacterization of the oligosaccharide component of arylsulfatase B from rat liver.
Author(s) -
Małgorzata Przybyło,
Anna Lityń́ska
Publication year - 1997
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1997_4412
Subject(s) - exoglycosidase , glycan , oligosaccharide , fucose , chemistry , arylsulfatase a , biochemistry , galactose , arylsulfatase , glycoconjugate , mannose , fucosylation , glycoprotein , enzyme
A glycan chain analysis of the total oligosaccharide pool derived from rat liver arylsulfatase B was carried out by. P4 Gel Permeation Chromatography and sequential exoglycosidase digestion. It was found that 71% of rat liver arylsulfatase B oligosaccharides were sialylated. The relative contribution of particular structures in the total glycan pool was as follows: sialylated biantennary complex type glycans with terminal galactose--65%, high-mannose type glycans--15%, biantennary complex type glycans with core fucose and terminal N-acetylglucosamine--5%, O-linked oligosaccharides--3.5%.