Open AccessCrystallization and preliminary crystallographic studies of a new crystal form of papain from Carica papaya.
Author(s) -
Maciej Kozak,
Elżbieta Kozian,
Zbigniew Grzonka,
M. Jaskólski
Publication year - 1997
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1997_4408
Subject(s) - papain , carica , monoclinic crystal system , crystallization , crystallography , crystal (programming language) , molecule , chemistry , crystal structure , x ray crystallography , materials science , diffraction , organic chemistry , botany , biology , physics , optics , computer science , programming language , enzyme
A new crystal form of papain from the latex of Carica papaya, complexed with an inhibitor (Z-Arg-Leu-Val-Gly-CHN2) was obtained by the vapor-diffusion method using a methanol/ethanol mixture as a precipitant. The slat-like crystals are monoclinic, space group P2(1), with unit cell parameters a = 42.6 A, b = 49.8 A, c = 50.5 A, A = 111.9 degrees, and contain one molecule in the asymmetric unit. The crystals are stable in the X-ray beam and diffract beyond 1.8 A. A molecular model has been placed in the unit cell by molecular replacement.