Open AccessInteraction of HIV Tat model peptides with tRNA and 5S rRNA.
Author(s) -
Małgorzata Giel-Pietraszuk,
Mirosława Z. Barciszewska,
Piotr Mucha,
Piotr Rekowski,
Gotfryd Kupryszewski,
Jan Barciszewski
Publication year - 1997
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1997_4407
Subject(s) - rna , transfer rna , ribosomal rna , biochemistry , rnase p , biology , dna , nucleotide , microbiology and biotechnology , chemistry , gene
New data are presented on the interaction of model synthetic peptides containing an arginine-rich region of human immunodeficiency virus (HIV-Tat), with native RNA molecules: tRNA(Phe) of Saccharomyces cerevisiae and 5S rRNA from Lupinus luteus. Both RNA species form complexes with the Tat1 (GRKKRRQRRRA) and Tat2 (GRKKRRQRRRAPQDSQTHQASLSKQPA) peptides, as shown by electrophoretic gel shift and RNase footprint assays, and CD measurements. The nucleotide sequence UGGG located in the dihydrouridine loop of tRNAPhe as well as in the loop D of 5S rRNA is specifically protected against RNases. Our data indicate direct interactions of guanine of RNA moieties with arginine residues. These interactions seem similar to those observed in DNA-protein complexes, but different from those previously observed in the TAR RNA-Tat complexes.