Open AccessExpression of small synthetic genes coding for hEGF, human epidermal growth factor, and CPTI II, serine proteinase inhibitor from Cucurbitacea, cloned in a novel expression/secretion vector in Saccharomyces cerevisiae.
Author(s) -
Jolanta M. Topczewska,
Bożenna Rempoła,
Magdalena Fikus
Publication year - 1996
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1996_4559
Subject(s) - yeast , expression vector , saccharomyces cerevisiae , secretion , cloning (programming) , gene , serine , biology , microbiology and biotechnology , epidermal growth factor , plasmid , biochemistry , recombinant dna , enzyme , receptor , computer science , programming language
Efficient synthesis of two small eukaryotic polypeptides of human and plant origin was carried out using a novel expression/secretion yeast vector, pYET. The yield was optimized in respect of the yeast strain, expression cassette construction, promoter regulation and culture conditions. Both cloned genes code for biotechnologically important proteins: human epidermal growth factor and a serine proteinase inhibitor from Cucurbitacea.