Open AccessNucleotide probes of DNA polymerases.
Author(s) -
George E. Wright
Publication year - 1996
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1996_4522
Subject(s) - dna polymerase , polymerase , nucleotide , dna clamp , dna polymerase ii , mutagenesis , dna , biochemistry , dna polymerase i , enzyme , chemistry , primase , microbiology and biotechnology , biology , polymerase chain reaction , mutation , reverse transcriptase , gene
The modified nucleotides, N2-(p-n-butylphenyl)dGTP and 2-(p-n-butylanilino) dATP and related compounds have been developed as inhibitor-probes of B family DNA polymerases. Synthetic approaches to these compounds are summarized. The nucleotides are potent, non-substrate inhibitors of DNA polymerase a. In contrast, they inhibit other members of the family with less potency but act as substrates for these enzymes. Modelling of the inhibitor: enzyme binding mechanism has been done based on the known structure of E. coli DNA polymerase I, and site-directed mutagenesis experiments to evaluate this mechanism are proposed.