Open AccessInteractions of rat repetitive sequence MspI8 with nuclear matrix proteins during spermatogenesis.
Author(s) -
Jacek Rogoliński,
Piotr Widłak,
Joanna Rzeszowska-Wolny
Publication year - 1996
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1996_4501
Subject(s) - nuclear matrix , spermatogenesis , nuclear protein , matrix (chemical analysis) , western blot , sequence (biology) , biology , microbiology and biotechnology , chemistry , genetics , gene , endocrinology , chromatin , chromatography , transcription factor
Using the Southwestern blot analysis we have studied the interactions between rat repetitive sequence MspI8 and the nuclear matrix proteins of rat testis cells. Starting from 2 weeks the young to adult animals showed differences in type of testis nuclear matrix proteins recognizing the MspI8 sequence. The same sets of nuclear matrix proteins were detected in some fractions enriched in spermatocytes and spermatides and obtained after fractionation of testis cells of adult animals by the velocity sedimentation technique.