Open AccessDNA adducts in environmental, occupational and life-style studies in human biomonitoring.
Author(s) -
Kari Hemminki,
Ewa Grzybowska,
Piotr Widłak,
M Chorazy
Publication year - 1996
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1996_4499
Subject(s) - adduct , dna , chemistry , carcinogen , dna damage , carcinogenesis , biomonitoring , dna adduct , environmental chemistry , biochemistry , organic chemistry , gene
The importance of DNA adducts in carcinogenesis had been discussed. The 32P-postlabelling method was developed as a quantitative technique to measure the level of different DNA adducts including adducts in human DNA. The elevated level of DNA adducts was found in white blood cells in persons exposed environmentally and occupationally to high concentrations of PAHs (polycyclic aromatic hydrocarbons) in the ambient air. Tobacco also generated higher level of DNA adducts both in lymphocytes and laryngeal tissues of smokers. Exposure to styrene has been of interest world-wide because of the very high exposure and persistence of adducts in DNA of lamination workers.