Open AccessThe use of sequential affinity chromatography for separation of human neutrophil elastase, cathepsin G and azurocidin.
Author(s) -
W Watorek,
Antoni Polanowski,
Tadeusz Wilusz
Publication year - 1996
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1996_4483
Subject(s) - cathepsin g , elastase , affinity chromatography , chemistry , neutrophil elastase , pancreatic elastase , trypsin , cathepsin , biochemistry , inflammation , enzyme , biology , immunology
Elastase, cathepsin G and azurocidin from human neutrophils are key components of body inflammatory defense. Perturbations in regulation of their activities lead to many serious pathological states. The paper describes a simple, fast and efficient method of joint purification of these proteins with the use of sequential affinity chromatography on squash trypsin inhibitor (CMTI I) and bovine pancreatic trypsin inhibitor (BPTI).