Open AccessGenetic variability of four natural isolates of the Stilpnotia salicis multiple-enveloped nuclear polyhedrosis virus.
Author(s) -
L. I. Strokovskaya,
J. Ziemnicka,
Joanna Michalik
Publication year - 1996
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1996_4459
Subject(s) - biology , nuclear polyhedrosis virus , genome , virology , restriction fragment length polymorphism , virus , genome size , nuclear dna , hindiii , dna , restriction fragment , genotype , genomic dna , genetics , restriction enzyme , gene , mitochondrial dna
Viral genome DNA from four different multiple-enveloped nuclear polyhedrosis virus isolates, obtained from naturally infected larvae of satin moth (Stilpnotia salicis), a pest of poplar tree (Populus) was analysed. Larvae were collected over a period of 11 years, from 1978 to 1989. The genomic DNA restriction patterns pointed to heterogeneity of these wild-type viruses. The differences observed in isolates of several years revealed limited restriction fragment length polymorphism and showed that these viruses contained distinct, but closely related genotypes. The genome size of SsMNPV was established as 128-134 kb, based on HindIII and SacI restriction analysis.