Open AccessInteraction of higher plant ribosomal 5S RNAs with Xenopus laevis transcriptional factor IIIA.
Author(s) -
Mirosława Z. Barciszewska
Publication year - 1994
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1994_4769
Subject(s) - xenopus , wheat germ , biology , ribosomal rna , microbiology and biotechnology , genetics , gene , biochemistry
In this paper transcriptional factor IIIA (TFIIIA) has been used as a probe for identity of three-dimensional-structure of eukaryotic 5S rRNAs. I was interested in finding a common motif in plant and Xenopus 5S rRNAs for TFIIIA recognition. I found that the two eukaryotic 5S rRNAs (from wheat germ and lupin seeds) are recognized by X. laevis TFIIIA and the data clearly suggest that these 5S rRNAs have very similar if not identical three-dimensional structures. Also effects of various conditions on stability of these complexes have been studied.