Open AccessDNA and proteins of the nuclear matrix are the main targets of benzo[a]pyrene's action in rat hepatocytes.
Author(s) -
Piotr Widłak,
Joanna Rzeszowska-Wolny
Publication year - 1993
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1993_4799
Subject(s) - nuclear matrix , benzo(a)pyrene , dna , carcinogen , chemistry , nuclear dna , pyrene , nuclear protein , microbiology and biotechnology , histone , matrix (chemical analysis) , adduct , biochemistry , biology , chromatin , gene , transcription factor , chromatography , organic chemistry , mitochondrial dna
The binding of [14C]benzo[a]pyrene (B[a]P) to DNA and proteins in total nuclei and subnuclear fractions of cultured rat hepatocytes was compared. The main targets of B[a]P were non-histone high molecular weight proteins of the nuclear matrix and DNA sequences attached to this structure. Following 24 h exposure to B[a]P the amounts of adducts in the nuclear matrix DNA and proteins were twice as high as in total nuclei. After withdrawal of the carcinogen containing medium the level of B[a]P-induced adducts gradually decreased but always remained the highest in the nuclear matrix proteins. Removal of adducts from the nuclear matrix DNA was more efficient than from the other DNA fractions, and 72 h after exposure to the carcinogen the level of DNA adducts in this fraction was similar to that in total nuclei.