Open AccessA study of the genotoxic potential of flavonoids using short-term bacterial assays.
Author(s) -
Hanna Czeczot,
Joanna Kusztelak
Publication year - 1993
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1993_4797
Subject(s) - isorhamnetin , apigenin , luteolin , chemistry , dna , ames test , quercetin , escherichia coli , biochemistry , flavonoid , salmonella , bacteria , biology , genetics , gene , antioxidant , kaempferol
Genotoxic activities of flavonoids (quercetin, rhamnetin, isorhamnetin, apigenin, luteolin) were investigated using two short-term bacterial assays. In the "repair test" in Salmonella typhimurium (strains TA1538 uvrB- and TA1978 uvrB+) the flavonoids studied did not introduce any damage into the DNA recognized by UvrABC nuclease (correndonuclease II). The results of the SOS-Chromotest in Escherichia coli K-12 strains PQ37 (tag+, alk+) and PQ243 (tagA, alkA) indicated that flavonoids only weakly induced the SOS system. The addition of a liver activation system (S9 mix) did not increase the mutagenic effect of the flavonoids tested. Two compounds: rhamnetin, isorhamnetin and their putative metabolites formed in the presence of the S9 mix did not alkylate DNA at N-3 of adenine.