Open AccessZ-RNA. The synthesis of 2'-O-[13C]methyl- and 5-methyl-analogs of ribo-CGCGCG.
Author(s) -
Ewa Biała,
Jan Milecki,
Andrzej Kowalewski,
Mariusz Popenda,
W. Z. Antkowiak,
Ryszard W. Adamiak
Publication year - 1993
Publication title -
acta biochimica polonica
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.452
H-Index - 78
eISSN - 1734-154X
pISSN - 0001-527X
DOI - 10.18388/abp.1993_4793
Subject(s) - phosphoramidite , guanosine , silylation , chemistry , reagent , derivative (finance) , rna , stereochemistry , carbon 13 nmr , organic chemistry , oligonucleotide , catalysis , biochemistry , dna , financial economics , economics , gene
Chemical synthesis of 2'-O-[13C]methyl-rCGCGCG and 5-methyl-rCGCGCG using support-aided phosphoramidite method is presented. 2'-O-Methyl guanosine derivative was separated from its 3'-O-methyl counterpart using transient 5',3'-O-silylation with 1,3-dichloro-1,1,3,3-tetraisopropyldisiloxane (Markiewicz reagent). The hexamers were obtained in a purity suitable for NMR studies.