z-logo
open-access-imgOpen Access
Biologically Active Terpenoids fromTamarix Species
Author(s) -
Н. А. Султанова,
Zharylkasyn A. Abilov,
A. K. Umbetova,
M. Iqbal Choudhary
Publication year - 2013
Publication title -
eurasian chemico-technological journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.227
H-Index - 9
eISSN - 2522-4867
pISSN - 1562-3920
DOI - 10.18321/ectj225
Subject(s) - chloroform , terpenoid , chemistry , ethyl acetate , ethanol , chromatography , stereochemistry , organic chemistry
We carried out research of bioactive substances obtained from ethanol extract (70%). This ethanol extract (70%) was obtained by us from the aerial parts of Tamarix plants (Т. laxa Willd., T. hispida Willd., T. ramosissima Ledeb. and Т. е longatа Ledeb.). These plants belong to the family of Tamaricaceae which grow on the territory of Kazakhstan. For the preliminary separation of bioactive substances, the fractional extraction of ethanol extracts (70%) of Tamarix species was carried out using chloroform and ethyl acetate. Chloroform and ethyl acetate extracts contained terpenoids (positive reactions with Lieberman-Burchard reagent and cerium sulfate). Isolation of terpenoids from chloroform and ethyl acetate extracts was achieved by a series of separations on chromatography column over silica gel and these terpenoids were eluted with gradient mixture of hexaneethylacetate (0-100%). From the aerial parts of Tamarix (Т. laxa Willd., T. hispida Willd., T. ramosissima Ledeb., Т. е longatа Ledeb) five terpenoids were isolated. Their structures of 1-5 were found using alkaline hydrolysis and UV, IR, 1 H and 13 C NMR, COSY-45°, HMQC, HMBC, EIMS, FABMS (-ve) spectral analyses. Pentacyclic triterpenoids: ursolic acid (1), 3β-al-D- friedoolean-14-en-28-oic acid methyl ether (2), 3-α-[3´´,4´´-dihydroxy-trans-cinnamoyloxy]-D-friedoolean-14-en-28-oic acids (isotamarixen) (3), 3-α-hydroxy-D-friedoolean-14-en-28-oic acid (4) and 3-α-[4´´-dihydroxy-trans-cinnamoyloxy]-Dfriedoolean -14-en-28-oic acid (5) were isolated from the aerial parts of Tamarix . Ethanol extracts (70%), chloroform, ethylacetate extracts from Tamarix laxa, Tamarix elongata showed antioxidant activity. Antioxidant activities were determined using the DPPH (1, 1-diphenyl-2-picrylhydrazyl radical) scavenging method. Ethanol extracts from Tamarix hispida (50%) were found to contain the triterpenoid: 3-α-[3´´, 4´´-dihydroxy-trans-cinnamoyloxy]-D-friedoolean-14-en-28-oic acids (isotamarixen), which were tested in vitro for their effect on the α-glycosidase enzyme activity. 3-α-[3´´, 4´´-dihydroxy-trans-cinnamoyloxy]-D-friedoolean-14-en-28-oic showed inhibiting effect on the α-glycosidase enzyme activity and was found to be a potent antioxidant.

The content you want is available to Zendy users.

Already have an account? Click here to sign in.
Having issues? You can contact us here