Open Access<i>In Vitro</i> Antioxidant and Anticancer Potency of <i>Tabernaemontana divaricata</i> (L.) Flowers
Author(s) -
C. Kalaimagal
Publication year - 2022
Publication title -
journal of natural remedies
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.154
H-Index - 19
eISSN - 2320-3358
pISSN - 0972-5547
DOI - 10.18311/jnr/2022/25367
Subject(s) - dpph , chemistry , radical , mtt assay , antioxidant , hela , hydrogen peroxide , nitric oxide , in vitro , viability assay , biochemistry , microbiology and biotechnology , biology , organic chemistry
A study was performed to identify an antioxidant and anticancer property of the flower of Tabernaemontana divaricata (L.). In vitro antioxidant activity was identified through the execution of various free radical scavenging assays like DPPH (1,1-diphenyl-2-picrylhydrazyl) radical, superoxide anion radical, nitric oxide radicals, hydrogen peroxide radicals in ethanolic flower extract of T. divaricata (L.). As a result of these scavenging methods, DPPH radicals were repressed at the percentage of 38.9% in 1000 ?g/ml. Likewise in the same concentration of extract, repression of superoxide anion radicals, nitric oxide radicals, and hydrogen peroxide radicals occurred at 49.3%, 56.4% and, 31.2% respectively. In vitro antiproliferative activity of the ethanolic flower extract was evaluated using 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT) assay to find the percentage of cell inhibition as well cell viability of HepG2 (Hepatocellular carcinoma), HeLa (Cervical adenocarcinoma) and A549 (Lung adenocarcinoma) cancer cell lines. Growth of all these cell lines was inhibited at 89.7%, 91.2% and 90.4% in 500 ?g/ml. MTT assay exhibited IC 50 value at 15.6 ?g/ml of extract concentration. The effect of plant extract concentration was identified through decreasing the percent of viable cancer cells when compared to control cells. The results showed that dose dependent cytotoxic activity of T. divaricata (L.) against all cancer cell lines. The present work concluded that flower of T. divaricata (L.) can act on various cancer cells under in vitro condition via neutralizing effect of free radicals.