Open AccessDevelopment and Validation of UV Spectrophotometric Method for Quantitative Estimation of Clobetasol 17-Propionate
Author(s) -
N. Anjali Devi,
Sunil Kumar,
Sunny Rajan,
Jagbir Gegoria,
Sheefali Mahant,
Rekha Rao
Publication year - 2016
Publication title -
asian journal of chemistry and pharmaceutical sciences
Language(s) - English
Resource type - Journals
ISSN - 2456-6217
DOI - 10.18311/ajcps/2016/7726
Subject(s) - clobetasol propionate , detection limit , chromatography , vitiligo , chemistry , accuracy and precision , correlation coefficient , solvent , psoriasis , mathematics , medicine , dermatology , statistics , organic chemistry
Clobetasol 17-propionate is used most potent topical glucocorticoid clinical effective in treatment of topical dermatitis, vitiligo and psoriasis. A rapid, simple, selective and precise UV- Visible Spectrophotometric method has been developed for the determination of Clobetasol 17-Propionate (CP) in bulk forms and dosage formulations. The spectrophotometric detection was carried out at an absorption maximum of 239 nm using ethanol as solvent. The method was validated for specificity, linearity, accuracy, precision, and robustness. The detector response for the CP was linear over the selected concentration range 2 to 40μg/ml with a correlation coefficient of 0.9999. The accuracy was between 99.1 and 101.4 %. The precision of 4μg/ml sample preparation three times in a day (intraday) was 0.1325%. The Limit of Detection (LOD) and Limit of Quantification (LOQ) are 0.84 and 2.55μg/ml, respectively. The recovery of CP was about 101.84%. The results demonstrated that the excipients in the commercial formulation did not interfere with the method and can be conveniently employed for daily routine quality control analysis of CP in bulk drug, marketed formulations.