Open AccessHistochemical Localization of Bio-macromolecules in Cestode (Raillietina sp.) infected country fowls (Gallus domesticus) in India
Author(s) -
Arkaprava Mandal,
N C Patra,
Saktipada Pradhan,
R N Hansda,
Samiran Mondal,
S. K. Mukhopadhayay,
DP Isore,
C.C. Dey
Publication year - 2021
Publication title -
indian journal of pathology and oncology
Language(s) - English
Resource type - Journals
eISSN - 2394-6792
pISSN - 2394-6784
DOI - 10.18231/j.ijpo.2021.095
Subject(s) - emaciation , fowl , eosin , biology , glycogen , staining , haematoxylin , histopathology , enteritis , gallus gallus domesticus , stain , veterinary medicine , alkaline phosphatase , pathology , microbiology and biotechnology , zoology , biochemistry , medicine , enzyme , paleontology , genetics
In Indian GDP, veterinary sector plays a major role in contribution wherein poultry meat plays good protein source. But the health status is less studied in case of country fowl. Indian country fowl are highly infected with producing emaciation, reduced growth rate, highly morbidity with enteritis. After confirmation by gross and microscopy, samples undergone histopathology study using Haematoxylin and Eosin stain (H&E). Thereafter, the samples also processed for detection of carbohydrate by McManus method using PAS stain and mucoprotein was detected using congo red method. Adult possesses carbohydrate, protein and fat. Histologically the mature, immature and gravid segments were differentiated whereas the histochemical techniques qualifies the biomacromolecules. The lipid droplet showed a clear vacuole preferably in and around the periphery portion. The glycogen distributed in an even manner. Subsequently, the protein was deposited mostly at the periphery in the body surfaces as well as in the egg.